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Overview
The Gyrasol HTS Sensor Reagent is a proprietary small molecule that contains a trivalent metal ion, which binds to phosphorylated biological substrates. Suitable substrates include peptides, lipids or DNA molecules that can be labeled with fluors such as fluorescein, TAMRA, Cy5 and derivatives. Phosphorylation is measured by the change of fluorescence of a dye-labeled and phosphorylated substrate when bound by the Sensor. The change in fluorescence directly correlates to the level of substrate conversion. When phospho-calibrators are included in the experimental setup, the precise amount of product formation can be determined.
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